LC-MS based global metabolite profiling of grapes: solvent extraction protocol optimisation

@article{Theodoridis2011LCMSBG,
  title={LC-MS based global metabolite profiling of grapes: solvent extraction protocol optimisation},
  author={Georgios Theodoridis and Helen I Gika and Pietro Franceschi and Lorenzo Caputi and Panagiotis Arapitsas and Mattias Scholz and Domenico Masuero and Ron Wehrens and Urska Vrhovsek and Fulvio Mattivi},
  journal={Metabolomics},
  year={2011},
  volume={8},
  pages={175-185}
}
Optimal solvent conditions for grape sample preparation were investigated for the purpose of metabolite profiling studies, with the aim of obtaining as many features as possible with the best analytical repeatability. Mixtures of water, methanol and chloroform in different combinations were studied as solvents for the extraction of ground grapes. The experimental design used a two stage study to find the optimum extraction medium. The extracts obtained were further purified using solid phase… CONTINUE READING
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