LC/MS guided isolation of alkaloids from lotus leaves by pH-zone-refining counter-current chromatography.

@article{Xu2011LCMSGI,
  title={LC/MS guided isolation of alkaloids from lotus leaves by pH-zone-refining counter-current chromatography.},
  author={Xia Xu and Cuirong Sun and Xiao-jing Dai and Rui-Lin Hu and Yuan-jiang Pan and Zhang-Fa Yang},
  journal={Molecules},
  year={2011},
  volume={16 3},
  pages={2551-60}
}
The traditional methods used in natural product separation primarily target the major components and the minor components may thus be lost during the separation procedure. Consequently, it's necessary to develop efficient methods for the preparative separation and purification of relatively minor bioactive components. In this paper, a LC/MS method was applied to guide the separation of crude extract of lotus (Nelumbo nucifera Gaertn.) leaves whereby a minor component was identified in the LC/MS… CONTINUE READING