L-type Ca2+ channel antagonists block voltage-dependent Ca2+ channels in identified leech neurons

  title={L-type Ca2+ channel antagonists block voltage-dependent Ca2+ channels in identified leech neurons},
  author={Paul Wilhelm Dierkes and Verena Wende and Peter Hochstrate and Wolf-R. Schlue},
  journal={Brain Research},

Ionic mechanism of ouabain-induced swelling of leech Retzius neurons

It is concluded that the ouabain-induced NaCl uptake is caused by the depolarization of the plasma membrane, which augments the inwardly directed electrochemical Cl− gradient.

Ca2+ influx into identified leech neurons induced by 5-hydroxytryptamine.

The effect of 5-HT on [Ca(2+)](i) (measured by Fura-2) in identified leech neurons under different experimental conditions, such as changed extracellular ion composition and blockade of excitatory synaptic transmission is investigated.

Role for calcium signaling and arachidonic acid metabolites in the activity-dependent increase of AHP amplitude in leech T sensory neurons.

The data indicate that a sophisticated cascade of intracellular signals links the repetitive discharge of T neurons to the activation of molecular pathways, which finally may alter the activity of critical enzymes such as the Na+/K+ ATPase, that sustains the generation of the AHP and its increase during repetitive stimulation.

Co-induction of LTP and LTD and its regulation by protein kinases and phosphatases.

The cellular properties of long-term potentiation following pairing of pre- and postsynaptic activity were examined at a known glutamatergic synapse in the leech, supporting the hypothesis that glutamate receptor trafficking contributes to both LTP and LTD at the P-to-AP synapse at the Leech.

In Esophageal Squamous Cells from Eosinophilic Esophagitis Patients, Th2 Cytokines Increase Eotaxin-3 Secretion through Effects on Intracellular Calcium and a Non-Gastric Proton Pump.

EoE cells express a non-gastric proton pump that mediates Th2 cytokine-stimulated eotaxin-3 secretion, suggesting a potential role for L-type calcium channel inhibitors in EoE treatment.

Pharmacological Strategies for Manipulating Plant Ca2+ Signalling

This review provides an overview of pharmacological strategies that are commonly used to activate or inhibit plant Ca2+ signalling, and focuses on highlighting modes of action where possible, and warn for potential pitfalls.

Ca2+ Dynamics in a Pollen Grain and Papilla Cell during Pollination of Arabidopsis1

Yellow cameleon was expressed in the pollen grains and papilla cells of Arabidopsis and monitored Ca2+ dynamics during pollination and remarkable increases in [Ca2+]cyt occurred three times in succession, just under the site of pollen-grain attachment.

Initial Variability and Time-Dependent Changes of Neuronal Response Features Are Cell-Type-Specific

It is concluded that the time-dependent change in excitability of T and P cells could indicate a cell-type-specific shift between different spiking regimes, which also could explain the high variability in the initial responses.

Ca 2 1 Dynamics in a Pollen Grain and Papilla Cell during Pollination of Arabidopsis 1

Ca 2 1 dynamics in the growing pollen tube have been well documented in vitro using germination assays and Ca 2 1 imaging techniques. However, very few in vivo studies of Ca 2 1 in the pollen grain



Na+, K+ and Ca2+ currents in identified leech neurones in culture.

The principal differences in membrane properties of identified leech neurones can be explained in terms of the numbers of Na+ channels and the distinctive kinetics of K+ channels in each type of cell.

Frequency and voltage-dependent inhibition of type IIA Na+ channels, expressed in a mammalian cell line, by local anesthetic, antiarrhythmic, and anticonvulsant drugs.

Dose-response relationships with phenytoin supported the hypothesis that the voltage dependence of tonic block resulted from the higher affinity of the drugs for inactivated than for resting channels, and type IIA Na+ channels expressed in a mammalian cell line retain the complex pharmacological properties characteristic of native Na+ channel.

Ca2+ currents in central insect neurons: electrophysiological and pharmacological properties.

The dihydropyridine insensitivity of the phenylalkylamine-sensitive HVA currents is a property the cockroach DUM cells share with other invertebrate neurons.

Distribution and functional properties of glutamate receptors in the leech central nervous system.

It is concluded that in leech segmental ganglia the majority of the neurons and the neuropile glial cells, but probably not the mechanosensory neurons, possess glutamate receptors of the AMPA-kainate type.

Functional characterization of ion permeation pathway in the N-type Ca2+ channel.

The results suggest that N-type Ca2+ channels share general features of a high affinity ion-binding site with the L-typeCa2+ channel, and that this site is easily accessible from the outside of the channel pore.