L-serine degradation in Escherichia coli K-12: cloning and sequencing of the sdaA gene.

  • H S Su, B. Franz Lang, E B Newman
  • Published 1989 in Journal of bacteriology

Abstract

A new mutant of Escherichia coli K-12 unable to grow with L-serine, glycine, and L-leucine has been isolated by lambda plac Mu insertion and shown to be deficient in L-serine deaminase activity. The corresponding gene, sdaA, has been cloned from a prototrophic strain, and the clone has been characterized and sequenced. The evidence is consistent with the hypothesis that sdaA is the structural gene for L-serine deaminase. However, other possibilities are also considered. No significant homology with previously reported DNA or protein sequences was detected.

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@article{Su1989LserineDI, title={L-serine degradation in Escherichia coli K-12: cloning and sequencing of the sdaA gene.}, author={H S Su and B. Franz Lang and E B Newman}, journal={Journal of bacteriology}, year={1989}, volume={171 9}, pages={5095-102} }