L-histidine functionalized multi-walled carbon nanotubes for on-line affinity separation and purification of immunoglobulin G in serum.

@article{Du2012LhistidineFM,
  title={L-histidine functionalized multi-walled carbon nanotubes for on-line affinity separation and purification of immunoglobulin G in serum.},
  author={Zhuo Du and Suling Zhang and Chanyuan Zhou and Miao Liu and Gongke Li},
  journal={Talanta},
  year={2012},
  volume={99},
  pages={40-9}
}
In this work, the multi-walled carbon nanotubes were covalently functionalized with L-histidine (His-MWNTs) as online pseudospecific affinity adsorbent for immunoglobulin G (IgG) separation and purification with a simple surface modification method, using 1-ethyl-3-(3-dimethyaminopropyl) carbodiimide hydrochloride (EDC) and N-hydroxysuccinimde (NHS). The affinity of the His-MWNTs toward IgG was investigated in a microcolumn incorporated into a sequential injection system, which also involves an… CONTINUE READING

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