L-citrulline production from L-arginine by macrophage nitric oxide synthase. The ureido oxygen derives from dioxygen.

@article{Kwon1990LcitrullinePF,
  title={L-citrulline production from L-arginine by macrophage nitric oxide synthase. The ureido oxygen derives from dioxygen.},
  author={Nyoun Soo Kwon and Carl Nathan and C D Gilker and Owen W. Griffith and Dwight E Matthews and Dennis J. Stuehr},
  journal={The Journal of biological chemistry},
  year={1990},
  volume={265 23},
  pages={13442-5}
}
Previously proposed mechanisms for the production of L-citrulline from L-arginine by macrophage nitric oxide (NO.) synthase involve either hydrolysis of arginine or hydration of an intermediate and thus predict incorporation of water oxygen into L-citrulline. Macrophage NO. synthase was incubated with L-arginine, NADPH, tetrahydrobiopterin, FAD, and dithiothreitol in H2(18)/16O2. L-Citrulline produced in this reaction was analyzed with gas chromatography/mass spectrometry. Its mass spectrum… CONTINUE READING

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