L-Sorbose phosphorylation in Escherichia coli K-12.

  title={L-Sorbose phosphorylation in Escherichia coli K-12.},
  author={Alex Slater and M. C. Jones-Mortimer and Hans Leo Kornberg},
  journal={Biochimica et biophysica acta},
  volume={646 2},
L-Sorbose is phosphorylated by Escherichia coli by two distinct Enzymes II of the phosphoenolpyruvate-dependent phosphotransferase system. The glucose Enzyme II (specified by the gene ptsG) phosphorylates L-sorbose with an apparent Km of 0.08 +/- 0.03 mM and V of 31.8 +/- 3.5 nmol . mg-1 . min-1 whilst the fructose Enzyme II (specified by the gene ptsF) phosphorylates it with an apparent Km of 28.9 +/- 2.7 mM and V of 20.2 +/- 0.8 nmol . mg-1 . min-1. L-Sorbose induces neither of these Enzymes… Expand
L-Sorbose metabolism in Klebsiella pneumoniae and Sor+ derivatives of Escherichia coli K-12 and chemotaxis toward sorbose
Transport and phosphorylation of L-sorbose was catalyzed by membrane-bound enzyme IIsor of the phosphoenolpyruvate-dependent carbohydrate:phosphotransferase system, specific for and regulated by this ketose and different from all other enzymes II described thus far. Expand
Construction of a new catabolic pathway for d-fructose in Escherichia coli K12 using an l-sorbose-specific enzyme from Klebsiella pneumoniae
A transport system and enzymes encoded in the fru and the mtl operons from E. coli K12 as well as in the sor operon from Klebsiella pneumoniae coding for an l-sorbose-1-phosphate reductase are presented. Expand
Molecular analysis of the phosphoenolpyruvate-dependent l-sorbose: phosphotransferase system from Klebsiella pneumoniae and of its multidomain structure
A 3.4 kb DNA fragment from the chromosome of Klebsiella pneumoniae that codes for a phosphoenolpyruvate-dependent l-sorbose: phosphotransferase system (PTS) is cloned and sequence comparisons revealed highly conserved structures for the membranebound proteins. Expand
High Throughput Screening Platform for a FAD-Dependent L-Sorbose Dehydrogenase
A reliable platform for high-throughput screening of more efficient FAD-dependent sorbose dehydrogenase (SDH) has been established and the titer of the 2-KLG has been improved by 14.1%. Expand
Maintenance of the cellobiose utilization genes of Escherichia coli in a cryptic state.
The observation that the same compound can serve either as a nutrient or as an inhibitor suggests that, under most conditions in which cellobiose will be present together with other resources, there is a strong selective advantage to having the cryptic (Cel0) allele. Expand
Cloning and physical mapping of the sor genes for L‐sorbose transport and metabolism from Klebsiella pneumoniae
The sor genes of Klebsiella pneumoniae KAY2026, which enable the bacterium to metabolize the ketose L‐sorbose, have been cloned on an 8.3kb DNA fragment into the multicopy plasmid, pACYC184. TheExpand
The Bacterial Phosphoenolpyruvate: Sugar Phosphotransferase System of Escherichia coli and Salmonella typhimurium
Bacteria such as Escherichia coli and Salmonella typhimurium can grow in simple defined media consisting of inorganic salts and any one of a wide variety of carbon sources. But the cells must be ableExpand
Positive and negative regulation of expression of the <Emphasis Type="SmallCaps">l</Emphasis>-sorbose (<Emphasis Type="Italic">sor</Emphasis>) operon by SorC in <Emphasis Type="Italic">Klebsiella pneumoniae</Emphasis>
In Klebsiella pneumoniae the gene products involved in the degradation of the ketose L-sorbose are encoded in the sor operon. It comprises, besides structural genes for uptake and catabolism, aExpand
Linkage Map of Escherichia coli K-12, Edition 10: The Traditional Map
  • M. Berlyn
  • Biology
  • Microbiology and Molecular Biology Reviews
  • 1998
This map is an update of the edition 9 map by Berlyn et al. and adds new genes discovered and established since 1996 and eliminates those shown to correspond to other known genes. Expand
Positive and negative regulation of expression of the l-sorbose (sor) operon by SorC in Klebsiella pneumoniae
The repressor activity of SorC was demonstrated by complementation of constitutive sorC alleles with a sorC+ allele leading to inducible expression of all sor genes, including sorC, which, as visualized by the use of a series of lacZ fusions, thus autoregulates its expression, both as an activator and a repressor. Expand


PtsX: A Gene involved in the uptake of glucose and fructose by Escherichia coli
Evidence is presented that the Enzyme II specified by the ptsX gene is involved also in the uptake and phosphorylation of glucose. Expand
The utilization of fructose by Escherichia coli. Properties of a mutant defective in fructose 1-phosphate kinase activity.
This mutant grew in media containing any one of a variety of substances, including hexoses, hexose 6-phosphates, sugar acids and glucogenic substrates, at rates not significantly different from those at which the parent organism grew on these substrates. Expand
Regulation of sugar accumulation by Escherichia coli
It is reported that the addition of pyruvate inhibits the accumulation of specific sugars by E. coli, and it is suggested that the inhibitory agent is acetyl-coenzyme A. Expand
Studies on the glucose-transport system in Escherichia coli with α-methylglucoside as substrate
Abstract The properties of the glucose-transport system of Escherichia coli K10 were studied by following intracellular accumulation of 14C-labeled α-methylglucoside. A mutant defective in the uptakeExpand
Purification and properties of rat liver fructokinase.
Abstract New spectrophotometric and radiochemical assay procedures for measuring fructokinase (ATP:d-fructose 1-phosphotransferase, EC activity are reported. This enzyme has been purifiedExpand
Xylitol and d-Arabitol Toxicities Due to Derepressed Fructose, Galactitol, and Sorbitol Phosphotransferases of Escherichia coli
  • A. Reiner
  • Medicine, Biology
  • Journal of bacteriology
  • 1977
D-Arabitol sensitivity also occurs in sorbitol-constitutive strains and is shown, like the previous two instances of pentitol toxicities, to result from a constitutive phosphotransferase, which is blocked in mutants selected for resistance. Expand
The mechanism by which glyceraldehyde inhibits glycolysis.
The conditions under which glyceraldehyde inhibition may be obtained are established and several important clues regarding the site of it,s action are uncovered but the mechanism by which this compound inhibits glycolysis has remained obscure. Expand
The ketokinase activity of the intestinal mucosa.
The heat-stable ketokinase has properties similar to the liver ketokin enzyme and its presence in the intestinal mucosa is compatible with the hypothesis that the mechanism of the transformation of fructose into glucose that takes place in theestinal mucosa might be essentially the same that operates in liver. Expand
La fructokinase du foie
Resume 1. 1. On a purifie a partir de foie de boeuf un enzyme qui catalyse la phosphorylation du fructose et du sorbose aux depends de l'ATP, mais qui est inactif vis-a-vis des aldohexoses et duExpand
Transduction of linked genetic characters of the host by bacteriophage P1.
Transduction of characters between bacteria of the coli and dysentery groups indicates genetic homologies between these groups. Expand