L-Cysteine Production in Escherichia coli Based on Rational Metabolic Engineering and Modular Strategy.

@article{Liu2018LCysteinePI,
  title={L-Cysteine Production in Escherichia coli Based on Rational Metabolic Engineering and Modular Strategy.},
  author={Han Liu and Guochen Fang and Hui Wu and Zhimin Li and Qin Ye},
  journal={Biotechnology journal},
  year={2018},
  volume={13 5},
  pages={
          e1700695
        }
}
L-cysteine is an amino acid with important physiological functions and has a wide range of applications in medicine, food, animal feed, and cosmetics industry. In this study, the L-cysteine synthesis in Escherichia coliEscherichia coli is divided into four modules: the transport module, sulfur module, precursor module, and degradation module. The engineered strain LH03 (overexpression of the feedback-insensitive cysE and the exporter ydeD in JM109) accumulated 45.8 mg L-1 of L-cysteine in 48 hr… CONTINUE READING