Knowing the glycosylation machinery of mammary gland cells : Potential and drawbacks for the expression of proteins of pharmaceutical interest

@inproceedings{Cremata2009KnowingTG,
  title={Knowing the glycosylation machinery of mammary gland cells : Potential and drawbacks for the expression of proteins of pharmaceutical interest},
  author={Jose A. Cremata and Raquel Montesino and Jorge Roberto Toledo and Oliberto S{\'a}nchez and Elsa Rodr{\'i}guez and Maria del Pilar Rodriguez Rodriguez},
  year={2009}
}
N-glycosylation analysis of recombinant human Erythropoietin (rhEPO) obtained by adenoviral transduction of goat mammary gland epithelial cells (GMGE) rhEPO-GMGE and in the milk of goats (rhEPO-Lc) was carried out by a combination of normal-phase HPLC (Amide-80) and ion exchange chromatography of the 4ABA labeled, enzymatically released N-glycans and… CONTINUE READING