Knockout of the Erythromycin Biosynthetic Cluster Gene, eryBI, Blocks Isoflavone Glucoside Bioconversion during Erythromycin Fermentations in Aeromicrobium erythreum but Not in Saccharopolyspora erythraea

@article{Reeves2008KnockoutOT,
  title={Knockout of the Erythromycin Biosynthetic Cluster Gene, eryBI, Blocks Isoflavone Glucoside Bioconversion during Erythromycin Fermentations in Aeromicrobium erythreum but Not in Saccharopolyspora erythraea},
  author={Andrew R. Reeves and Ramya Seshadri and Igor A. Brikun and William H. Cernota and Melissa C. Gonzalez and J. Mark Weber},
  journal={Applied and Environmental Microbiology},
  year={2008},
  volume={74},
  pages={7383 - 7390}
}
ABSTRACT Isoflavone glucosides are valuable nutraceutical compounds and are present in commercial fermentations, such as the erythromycin fermentation, as constituents of the soy flour in the growth medium. The purpose of this study was to develop a method for recovery of the isoflavone glucosides as value-added coproducts at the end of either Saccharopolyspora erythraea or Aeromicrobium erythreum fermentation. Because the first step in isoflavone metabolism was known to be the conversion of… 
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Biotransformation and recovery of the isoflavones genistein and daidzein from industrial antibiotic fermentations
The objective of this study was to follow the metabolic fate of isoflavone glucosides from the soybean meal in a model industrial fermentation to determine if commercially useful isoflavones could be…
Identification and Characterization of a New Erythromycin Biosynthetic Gene Cluster in Actinopolyspora erythraea YIM90600, a Novel Erythronolide-Producing Halophilic Actinomycete Isolated from Salt Field
Erythromycins (Ers) are clinically potent macrolide antibiotics in treating pathogenic bacterial infections. Microorganisms capable of producing Ers, represented by Saccharopolyspora erythraea, are…
Fermentation optimization and industrialization of recombinant Saccharopolyspora erythraea strains for improved erythromycin a production
TLDR
Analysis of intra- and extracellular metabolites and key enzyme activities in central carbon metabolism revealed that the pool of TCA cycle intermediates was enhanced by the addition of corn steep liquor and induced an increase in erythromycin biosynthesis.
Release of Soybean Isoflavones by Using a β‐Glucosidase from Alicyclobacillus herbarius
TLDR
A novel β‐glycosidase from the acidophilic organism Alicyclobacillus herbarius was cloned and heterologously expressed in Escherichia coli BL21(DE3), which exhibited excellent tolerance to fructose and good tolerance to glucose and tolerated organic solvents.
Improved heterologous erythromycin A production through expression plasmid re‐design
TLDR
Improvements to final production were made by altering the design of the expression plasmids needed for biosynthetic pathway introduction, which led to improved final compound titers and suggest additional forms of pathway engineering to further improve results from heterologous production attempts.
A β-(1,2)-glycosynthase and an attempted selection method for the directed evolution of glycosynthases.
TLDR
The results from the attempted selection method provide insight into the requirements for in vivo directed evolution of glycosynthases, and the first biochemical characterization of the EryBI protein is provided.
Genome Sequence of Aeromicrobium erythreum NRRL B-3381, an Erythromycin-Producing Bacterium of the Nocardioidaceae
TLDR
Aeromicrobium erythreum NRRL B-3381 has a 3,629,239-bp circular genome that has 72% G+C content and provides resources for metabolic and combinatorial engineering of polyketides.
Metabolic engineering of antibiotic-producing actinomycetes using in vitro transposon mutagenesis.
TLDR
In this chapter, a detailed tagged Mutagenesis approach is described using in vitro transposon mutagenesis which allowed the successful identification of key genes involved in macrolide (erythromycin) antibiotic biosynthesis.

References

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