Knocking out multi-gene redundancies via cycles of sexual assortment and fluorescence selection

  title={Knocking out multi-gene redundancies via cycles of sexual assortment and fluorescence selection},
  author={Yo Suzuki and Robert P St Onge and Ramamurthy Mani and Oliver D. King and Adrian M. Heilbut and Vyacheslav M Labunskyy and Weidong Chen and Linda A. Pham and Lan V. Zhang and Amy Hin Yan Tong and Corey Nislow and Guri Giaever and Vadim N. Gladyshev and Marc Vidal and Peter W Schow and Joseph Lehar and Frederick P. Roth},
  booktitle={Nature Methods},
Phenotypes that might otherwise reveal a gene's function can be obscured by genes with overlapping function. This phenomenon is best known within gene families, in which an important shared function may only be revealed by mutating all family members. Here we describe the 'green monster' technology that enables precise deletion of many genes. In this method, a population of deletion strains with each deletion marked by an inducible green fluorescent protein reporter gene, is subjected to… CONTINUE READING
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