Knockdown expression of IL-10Rα gene inhibits PRRSV replication and elevates immune responses in PBMCs of Tibetan pig in vitro

@article{Chen2017KnockdownEO,
  title={Knockdown expression of IL-10Rα gene inhibits PRRSV replication and elevates immune responses in PBMCs of Tibetan pig in vitro},
  author={Yihui Chen and Ye Wang and Kai Zeng and Yun-feng Lei and Xiaohui Chen and San-cheng Ying and Xue-Bin Lv and Zezhou Wang and Rong Gao},
  journal={Veterinary Research Communications},
  year={2017},
  pages={1-8}
}
Increase of interleukin-10 (IL-10) induced by Porcine Reproductive and Respiratory Syndrome Virus (PRRSV) infection has been intensely studied to inhibit the anti-viral responses of host pigs. Blockade of expression of IL-10 receptor (IL-10R) by RNA interference (RNAi) may relieve the immunosuppression caused by excessive IL-10 in PRRSV infection. The recombinant short hairpin expressing plasmid targeted to pig IL-10Rα was transfected into peripheral blood mononuclear cells of Tibetan pig (Tp… CONTINUE READING