Knock-out mutants from an En-1 mutagenized Arabidopsis thaliana population generate phenylpropanoid biosynthesis phenotypes.

@article{Wisman1998KnockoutMF,
  title={Knock-out mutants from an En-1 mutagenized Arabidopsis thaliana population generate phenylpropanoid biosynthesis phenotypes.},
  author={Ellen Wisman and Ulrike Hartmann and Martin Sagasser and Elvira Baumann and Klaus Palme and K. -H. Hahlbrock and Heinz Saedler and Bernd Weisshaar},
  journal={Proceedings of the National Academy of Sciences of the United States of America},
  year={1998},
  volume={95 21},
  pages={12432-7}
}
A collection of 8,000 Arabidopsis thaliana plants carrying 48,000 insertions of the maize transposable element En-1 has been generated. This population was used for reverse genetic analyses to identify insertions in individual gene loci. By using a PCR-based screening protocol, insertions were found in 55 genes. En-1 showed no preference for transcribed or untranscribed regions nor for a particular orientation relative to the gene of interest. In several cases, En-1 was inserted within a few… CONTINUE READING
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