Kluyveromyces lactis LAC4 promoter variants that lack function in bacteria but retain full function in K. lactis.

@article{Colussi2005KluyveromycesLL,
  title={Kluyveromyces lactis LAC4 promoter variants that lack function in bacteria but retain full function in K. lactis.},
  author={Paul A. Colussi and Christopher H. Taron},
  journal={Applied and environmental microbiology},
  year={2005},
  volume={71 11},
  pages={
          7092-8
        }
}
The strong LAC4 promoter (P(LAC4)) from Kluyveromyces lactis has been extensively used to drive expression of heterologous proteins in this industrially important yeast. A drawback of this expression method is the serendipitous ability of P(LAC4) to promote gene expression in Escherichia coli. This can interfere with the process of assembling expression constructs in E. coli cells prior to their introduction into yeast cells, especially if the cloned gene encodes a protein that is detrimental… CONTINUE READING

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