Kinetics of transit of transferrin and epidermal growth factor through clathrin-coated membranes.

@article{Hanover1984KineticsOT,
  title={Kinetics of transit of transferrin and epidermal growth factor through clathrin-coated membranes.},
  author={John A Hanover and Mark C. Willingham and Ira Pastan},
  journal={Cell},
  year={1984},
  volume={39 2 Pt 1},
  pages={283-93}
}
The kinetics of association of epidermal growth factor (EGF) and diferric-transferrin (TF) with clathrin-coated membranes of KB cells were examined using anti-clathrin antibody bound to Staphylococcus aureus. The ligands were bound to cells at 4 degrees C and after warming to 30 degrees C for 5 min, both ligands were found concentrated in a membrane fraction immunoadsorbed with anti-clathrin antibody. Fifteen minutes after entry both ligands moved to a non-clathrin-associated compartment… CONTINUE READING

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