Kinetics of the Na+-H+ antiporter as assessed by the change in intracellular pH in MDCK cells.

@article{Selvaggio1986KineticsOT,
  title={Kinetics of the Na+-H+ antiporter as assessed by the change in intracellular pH in MDCK cells.},
  author={A M Selvaggio and John H. Schwartz and Howard H. Bengele and E Alexenskii Alexander},
  journal={The American journal of physiology},
  year={1986},
  volume={251 4 Pt 1},
  pages={
          C558-62
        }
}
The Na+-H+ antiporter regulates both H+ secretion and cell pH in renal epithelia. The present study was designed to further define the Na+ dependency and kinetics of proton efflux in MDCK cells. Intracellular pH (pHi) was determined with a pH-sensitive fluorescent probe, 2,7-bis-carboxyethyl-5,6-carboxyfluorescein (BCECF). Data were obtained from confluent monolayers grown on plastic cover slips and studied in media free of added CO2 and HCO-3, pH = 7.2 (pHo). Monolayers in NaCl maintain a pHi… CONTINUE READING
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