Kinetics of prostacyclin synthesis in PGHS-1-overexpressed endothelial cells.

@article{Sanduja1994KineticsOP,
  title={Kinetics of prostacyclin synthesis in PGHS-1-overexpressed endothelial cells.},
  author={Sudershan K. Sanduja and Ah-lim Tsai and Nevenka Matijevic-Aleksic and Kenneth K. Wu},
  journal={The American journal of physiology},
  year={1994},
  volume={267 5 Pt 1},
  pages={
          C1459-66
        }
}
The availability of a human endothelial cell overexpressed with prostaglandin H synthase-1 (PGHS-1) by retrovirus-mediated gene transfer made it possible to quantify the kinetics of prostacyclin [prostaglandin (PG)I2] synthesis and PGHS-1 turnover. Prostacyclin synthesis in response to arachidonate (AA) and ionophore A-23187 fit a single exponential kinetics. The rate constants for AA- and ionophore-treated cells were 0.064 min-1 [half-life (t1/2) of 11 min] and 0.032 min-1 (t1/2 = 22 min… 
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