Kinetics of methylation of DNA by a restriction endonuclease from Escherichia coli B.

@article{Vovis1974KineticsOM,
  title={Kinetics of methylation of DNA by a restriction endonuclease from Escherichia coli B.},
  author={G. F. Vovis and K Horiuchi and Norton D. Zinder},
  journal={Proceedings of the National Academy of Sciences of the United States of America},
  year={1974},
  volume={71 10},
  pages={3810-3}
}
The restriction endonuclease from E. coli B is both an endonuclease and a DNA methylase. Both activities either require or are stimulated by Mg(+2), adenosine triphosphate, and S-adenosyl-L-methionine. The particular activity which the enzyme exhibits depends upon the nature of the SB sites, the genetic sites that identify substrate DNA. Enzymatic treatment of DNA that has an unmodified, wild-type SB site results in either rapid restriction of the DNA or very slow methylation of the SB site. On… CONTINUE READING

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Genetic control of restriction and modification in Escherichia coli

M. Meselson, R. Yuan, J. Heywood
1964

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