Kinetics of inhibition and molecular asymmetry in pigeonpea (Cajanus cajan) urease.

  title={Kinetics of inhibition and molecular asymmetry in pigeonpea (Cajanus cajan) urease.},
  author={Punit Kumar Srivastava and Arvind Mohan Kayastha and Medicherla Venkata Jagannadham},
  journal={Journal of biochemistry, molecular biology, and biophysics : JBMBB : the official journal of the Federation of Asian and Oceanian Biochemists and Molecular Biologists},
  volume={6 1},
Urease from seeds of pigeonpea showed a time-dependent and irreversible inactivation at very low concentrations of heavy metal ions. Concentration of Cu(2+), Hg(2+) and Ag(+) required for 50% inactivation, on 10 min of incubation, were found to be 2.2 x 10(-6), 2.9 x 10(-8) and 6.3 x 10(-12) M, respectively. The kinetics of inactivation with each of these metal ions was found to be biphasic, with half of the activity being lost in a fast phase and remaining in a slow phase. Acetohydroxamate… CONTINUE READING