Kinetics of epidermal growth factor/receptor binding on cells measured by total internal reflection/fluorescence recovery after photobleaching

@article{Hellen1991KineticsOE,
  title={Kinetics of epidermal growth factor/receptor binding on cells measured by total internal reflection/fluorescence recovery after photobleaching},
  author={Edward H. Hellen and Daniel Axelrod},
  journal={Journal of Fluorescence},
  year={1991},
  volume={1},
  pages={113-128}
}
Total internal reflection fluorescence (TIRF) microscopy is used to measure the dissociation kinetic rate of fluorescein-labeled epidermal growth factor from its specific receptors on the surface of intact but mildly fixed A431 human epidermoid cells in culture. Prior applications of TIRF microscopy have been limited to nonreceptor binding or to model… CONTINUE READING