Kinetics of degradation of "short-" and "long-lived" proteins in cultured mammalian cells.

  title={Kinetics of degradation of "short-" and "long-lived" proteins in cultured mammalian cells.},
  author={Denys N. Wheatley and Michael Giddings and M S Inglis},
  journal={Cell biology international reports},
  volume={4 12},
Two distinct classes of protein referred to as short- and long-lived (Poole and Wibo, 1973) were found in pulse-chased HeLa S-3 and BHK 21/C13 cells. From experiments with pulse times ranging from 1 min to 20 h, a clear inverse correlation emerged between the pulse length and the percentage of protein which was hydrolysed intracellularly in the first h of chase. Using a 5 min pulse labelling with 3H-leucine, cells were either harvested immediately or after a 2 h chase. Approximately 35% of the… CONTINUE READING

From This Paper

Topics from this paper.


Publications citing this paper.
Showing 1-10 of 30 extracted citations

Similar Papers

Loading similar papers…