Kinetics of comet formation in single-cell gel electrophoresis: loops and fragments.

@article{Afanasieva2010KineticsOC,
  title={Kinetics of comet formation in single-cell gel electrophoresis: loops and fragments.},
  author={Katerina Afanasieva and Marianna Zazhytska and Andrei Sivolob},
  journal={Electrophoresis},
  year={2010},
  volume={31 3},
  pages={512-9}
}
We investigated the mechanisms of DNA exit during single-cell gel electrophoresis (the comet assay) by measuring the kinetics of the comet tail formation. In the neutral comet assay, the rate of DNA exit was found to be dependent on the topological state of DNA, which was influenced by either ethidium bromide or a low radiation dose. The results clearly show that the comet tail is formed by extended DNA loops: the loop extension, being reversible when the DNA torsional constraint remains in the… CONTINUE READING

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