Kinetics of colchicine binding to purified beta-tubulin isotypes from bovine brain.

@article{Banerjee1992KineticsOC,
  title={Kinetics of colchicine binding to purified beta-tubulin isotypes from bovine brain.},
  author={Asok Banerjee and Richard F. Luduena},
  journal={The Journal of biological chemistry},
  year={1992},
  volume={267 19},
  pages={13335-9}
}
Tubulin, the constituent protein of microtubules, is an alpha beta heterodimer; both alpha and beta exist in several isotypic forms whose functional significance is not precisely known. The antimitotic alkaloid colchicine binds to mammalian brain tubulin in a biphasic manner under pseudo-first-order conditions in the presence of a large excess of colchicine… CONTINUE READING