Kinetics of actin elongation and depolymerization at the pointed end.

@article{Weber1987KineticsOA,
  title={Kinetics of actin elongation and depolymerization at the pointed end.},
  author={Annemarie Weber and Jobeth Northrop and Marilyn Frances Bishop and Frank A. Ferrone and Mark S. Mooseker},
  journal={Biochemistry},
  year={1987},
  volume={26 9},
  pages={
          2537-44
        }
}
We measured the rate of elongation at the pointed filament end with increasing concentrations of G-actin [J(c) function] using villin-capped actin filaments of very small (actin/villin = 3, VA3) and relatively large size (actin/villin = 18, VA18) as nuclei for elongation. The measurements were made under physiological conditions in the presence of both Mg2+ and K+. In both cases the J(c) function was nonlinear. In contrast to the barbed filament end, however, the slope of the J(c) function… CONTINUE READING