Kinetics of Nif gene expression in a nitrogen-fixing bacterium.

  title={Kinetics of Nif gene expression in a nitrogen-fixing bacterium.},
  author={C{\'e}sar Poza-Carri{\'o}n and Emilio Jim{\'e}nez-Vicente and M{\'o}nica Navarro-Rodr{\'i}guez and Carlos Echavarri-Erasun and Luis M Rubio},
  journal={Journal of bacteriology},
  volume={196 3},
Nitrogen fixation is a tightly regulated trait. Switching from N2 fixation-repressing conditions to the N2-fixing state is carefully controlled in diazotrophic bacteria mainly because of the high energy demand that it imposes. By using quantitative real-time PCR and quantitative immunoblotting, we show here how nitrogen fixation (nif) gene expression develops in Azotobacter vinelandii upon derepression. Transient expression of the transcriptional activator-encoding gene, nifA, was followed by… CONTINUE READING