Kinetics of CO binding to putative Na(+)-motive oxidases of the o-type from Bacillus FTU and of the d-type from Escherichia coli.

Abstract

The kinetics of CO reassociation with isolated Bacillus FTU o-type oxidase and with solubilized membranes of Escherichia coli (GO102 strain) containing the d-type oxidase only, upon laser flash photolysis under reducing conditions, were studied. In both cases, kinetics are shown to be composed of three phases (tau 35-70 microseconds, 0.25-0.5 ms and 2-5 ms… (More)

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