Kinetics and thermodynamics of the interaction of 1-anilino-naphthalene-8-sulfonate with proteins.

@article{Cattoni2009KineticsAT,
  title={Kinetics and thermodynamics of the interaction of 1-anilino-naphthalene-8-sulfonate with proteins.},
  author={Diego I Cattoni and Sergio B Kaufman and F Luis Gonz{\'a}lez Flecha},
  journal={Biochimica et biophysica acta},
  year={2009},
  volume={1794 11},
  pages={1700-8}
}
Although 1-anilino-naphthalene-8-sulfonate (ANS) has been widely used in protein folding and binding studies, the detailed mechanism of this interaction is not fully understood. In this work the binding of ANS was analyzed at pre-equilibrium and equilibrium conditions using bovine serum albumin (BSA) as model. We employed a combined approach including the analysis of fluorescence, near-UV circular dichroism and isothermal titration calorimetric data. Experiments at equilibrium with these… CONTINUE READING