Corpus ID: 10462938

Kinetics and properties of beta-ketothiolase from Clostridium pasteurianum.

@article{Berndt1975KineticsAP,
  title={Kinetics and properties of beta-ketothiolase from Clostridium pasteurianum.},
  author={H. Berndt and H. Schlegel},
  journal={Archives of microbiology},
  year={1975},
  volume={103 1},
  pages={
          21-30
        }
}
1. Beta-Ketothiolase of Clostridium pasteurianum was purified 130-fold by ammonium sulphate fractionation and by column chromatography using DEAE-Sephadex A-50 and hydroxylapatite. Subjected to gel electrophoresis beta-ketothiolase revealed two distinct bands; by isoelectric focusing two enzymes with isoelectric points at pH 4.5 and 7.6 were separated. As established by sucrose density gradient centrifugation the molecular weight of both enzymes was found to be 158000. 2. The condensation… Expand
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