Kinetics and mechanisms of activation and inhibition of porcine liver fructose-1,6-bisphosphatase by monovalent cations.

@article{Zhang1996KineticsAM,
  title={Kinetics and mechanisms of activation and inhibition of porcine liver fructose-1,6-bisphosphatase by monovalent cations.},
  author={Rong-gaung Zhang and V Villeret and William N. Lipscomb and Herbert J. Fromm},
  journal={Biochemistry},
  year={1996},
  volume={35 9},
  pages={3038-43}
}
K+ and Li+ were used to study the kinetic effects of monovalent cations on porcine liver fructose-1,6-bisphosphatase (FBPase). At saturating fructose 1,6-bisphosphate (FBP) concentrations, Li+ was found to be a linear noncompetitive inhibitor with respect to Mg2+. K+ was found to activate the wild-type enzyme at low concentrations (K(m) = 17 mM) and to inhibit the enzyme at high concentrations (K(IK+) = 68mM). A steady-state random ter mechanism was proposed, and a mathematical equation was… CONTINUE READING