Kinetics and mechanism of the folding of cytochrome c.

  title={Kinetics and mechanism of the folding of cytochrome c.},
  author={Kenneth M. Pryse and T G Bruckman and B W Maxfield and Elliot L. Elson},
  volume={31 22},
The reversible folding of cytochrome c in urea at pH 4.0 was investigated by repetitive pressure perturbation kinetics and by equilibrium spectroscopic methods. Two folding reactions were observed in the 1 ms to 10 s time range. The rates and amplitudes of these reactions depend on urea concentration in a complex manner, which is different for each process. The absorbance spectra of the kinetic amplitudes of the two reactions also differ from each other. A model with a three-state mechanism can… CONTINUE READING