Kinetics and mechanism of Ca2+ binding to arsenazo III and antipyrylazo III.

  title={Kinetics and mechanism of Ca2+ binding to arsenazo III and antipyrylazo III.},
  author={P. Dorogi},
  journal={Biochimica et biophysica acta},
  volume={799 1},
  • P. Dorogi
  • Published 1984
  • Chemistry, Medicine
  • Biochimica et biophysica acta
Equilibrium and temperature-jump spectrophotometric measurements were carried out on arsenazo III (Ar) and antipyrylazo III (Ap) in order to establish the kinetic reaction schemes for complexing of these dyes with Ca2+. The reaction media contained 30 mM Na2HPO4 as the buffer salt, at pH 7.4. Dependence of the relaxation rate of arsenazo III on dye and Ca2+ concentrations indicates the presence of both CaAr and CaAr2 complexes, with the CaAr2 form being responsible for the slow, 10-20 ms… Expand
4 Citations
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Affinity and stoichiometry of calcium binding by arsenazo III.
  • P. Bauer
  • Chemistry, Medicine
  • Analytical biochemistry
  • 1981
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