Kinetics and crystal structure of human purine nucleoside phosphorylase in complex with 7-methyl-6-thio-guanosine.

@article{Silva2005KineticsAC,
  title={Kinetics and crystal structure of human purine nucleoside phosphorylase in complex with 7-methyl-6-thio-guanosine.},
  author={Rafael Gudim da Silva and Jos{\'e} Henrique Pereira and Fernanda Canduri and Walter Filgueira de Azevedo and Luiz Augusto Basso and Di{\'o}genes Santiago Santos},
  journal={Archives of biochemistry and biophysics},
  year={2005},
  volume={442 1},
  pages={49-58}
}
Purine nucleoside phosphorylase (PNP) catalyzes the reversible phosphorolysis of nucleosides and deoxynucleosides, generating ribose 1-phosphate and the purine base, which is an important step of purine catabolism pathway. The lack of such an activity in humans, owing to a genetic disorder, causes T-cell impairment, and drugs that inhibit this enzyme may have the potential of being utilized as modulators of the immunological system to treat leukemia, autoimmune diseases, and rejection in organ… CONTINUE READING