Kinetic study of the reaction of glutathione peroxidase with peroxynitrite.

@article{Briviba1998KineticSO,
  title={Kinetic study of the reaction of glutathione peroxidase with peroxynitrite.},
  author={Karlis Briviba and Reinhard Kissner and Willem H Koppenol and Helmut Sies},
  journal={Chemical research in toxicology},
  year={1998},
  volume={11 12},
  pages={1398-401}
}
Glutathione peroxidases and their mimics, e.g., ebselen or diaryl tellurides, efficiently reduce peroxynitrite/peroxynitrous acid (ONOO-/ONOOH) to nitrite and protect against oxidation and nitration reactions. Here, we report the second-order rate constant for the reaction of the reduced form of glutathione peroxidase (GPx) with peroxynitrite as (8.0 +/- 0.8) x 10(6) M-1 s-1 (per GPx tetramer) at pH 7.4 and 25 degreesC. The rate constant for oxidized GPx is about 10 times lower, (0.7 +/- 0.2) x… CONTINUE READING