Kinetic studies on the formation of estrogens from dehydroepiandrosterone sulfate by human placental microsomes.

@article{Gibb1984KineticSO,
  title={Kinetic studies on the formation of estrogens from dehydroepiandrosterone sulfate by human placental microsomes.},
  author={William F. Gibb and Julye C. Lavoie},
  journal={Endocrinology},
  year={1984},
  volume={114 6},
  pages={
          2323-9
        }
}
Using microsomes isolated from term human placentae kinetic analyses of each of the enzymes involved in estrogen synthesis from dehydroepiandrosterone sulfate have been carried out and the following parameters were found: sulfatase, Michaelis-Menten constant (Km) = 16,000 +/- 5,000 nM, maximum velocity (Vm) = 2.0 +/- 0.5 nmol X min-1 X mg protein-1; 3 beta-hydroxysteroid dehydrogenase (3 beta-HSD), Km = 15 +/- 3 nM, Vm = 1.8 +/- 0.4 nmol X min-1 X mg protein-1; aromatase, Km = 14 +/- 4 nM, Vm… CONTINUE READING