Kinetic studies of the demetallization and inactivation of concanavalin A.

The demetallization of various metallo derivatives of Concanavalin A (i.e., MnMnPL, CoMnPL, CaCaPL, CoCaPL and MnCaPL, where PL represents protein in a locked conformation) has been examined by three separate procedures. These include the treatment of the protein with the metal ion chelators, EDTA and terpyridine, and subjecting the protein to low pH (i.e… CONTINUE READING