Kinetic studies of ferrous ion oxidation with crystalline human ferroxidase (ceruloplasmin).

@article{Osaki1966KineticSO,
  title={Kinetic studies of ferrous ion oxidation with crystalline human ferroxidase (ceruloplasmin).},
  author={Shigemasa Osaki},
  journal={The Journal of biological chemistry},
  year={1966},
  volume={241 21},
  pages={5053-9}
}
The kinetics of the catalysis of Fe(I1) oxidation by crystalline human ferroxidase (ceruloplasmin) has been studied under various conditions at 30’. Two Km values with respect to Fe(U), Km1 = 0.6 /.tM and Km2 = 50 PM, were obtained at pH 6.5, at which ferroxidase gave the maximum activity of 550 Fe(I1) per ferroxidase per min in an acetate buffer. Competition between the two substrates, p-phenylenediamine and Fe(II), was observed. The stoichiometry of Fe(U), ferroxldase, and oxygen, determined… CONTINUE READING
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hp://w w w .jb.org/ D ow nladed from Shigemasa Osaki (Ceruloplasmin) Kinetic Studies of Ferrous Ion Oxidation with Crystalline Human Ferroxidase 1966, 241:5053-5059

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