Kinetic studies of a soluble alpha beta complex of nitrate reductase A from Escherichia coli. Use of various alpha beta mutants with altered beta subunits.

@article{Buc1995KineticSO,
  title={Kinetic studies of a soluble alpha beta complex of nitrate reductase A from Escherichia coli. Use of various alpha beta mutants with altered beta subunits.},
  author={Jean Buc and C L Santini and F. Blasco and Roger Giordani and Mar{\'i}a Luz C{\'a}rdenas and Marc Chippaux and Athel Cornish-Bowden and G{\'e}rard Giordano},
  journal={European journal of biochemistry},
  year={1995},
  volume={234 3},
  pages={
          766-72
        }
}
A soluble alpha beta complex of nitrate reductase can be obtained from a strain of Escherichia coli that lacks the narI gene and expresses only the alpha and beta subunits. The beta subunit contains four Fe-S centres and the alpha subunit contains the molybdenum cofactor, which is the site at which nitrate is reduced. Despite the lack of the gamma subunit of the complete enzyme, this complex can still catalyse the reduction of nitrate with artificial electron donors such as benzyl viologen, so… CONTINUE READING

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