Kinetic studies of Fos.Jun.DNA complex formation: DNA binding prior to dimerization.

@article{Kohler2001KineticSO,
  title={Kinetic studies of Fos.Jun.DNA complex formation: DNA binding prior to dimerization.},
  author={John M J Kohler and Alanna Schepartz},
  journal={Biochemistry},
  year={2001},
  volume={40 1},
  pages={130-42}
}
The bZIP proteins Fos and Jun bind DNA rapidly and with high affinity, forming a heteromeric complex that mediates activated transcription. Here we use stopped-flow fluorescence resonance energy transfer (FRET) to study the kinetic pathway by which Fos.Jun. DNA complexes assemble. Though dimerization of Fos and Jun occurs rapidly in the absence of DNA, the rate of dimerization is enhanced in the presence of DNA. Global analysis of the kinetic data shows that the favored DNA binding pathway is… CONTINUE READING

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