Kinetic properties of the redox switch/redox coupling mechanism as determined in primary cultures of cortical neurons and astrocytes from rat brain.

@article{Ramrez2007KineticPO,
  title={Kinetic properties of the redox switch/redox coupling mechanism as determined in primary cultures of cortical neurons and astrocytes from rat brain.},
  author={Bel{\'e}n G Ram{\'i}rez and Tiago B Rodrigues and In{\^e}s R Violante and F Cruz and Lu{\'i}s L. Fonseca and Paloma Ballesteros and M. Margarida C. A. Castro and Mar{\'i}a L. Garcia-Martin and Sebasti{\'a}n Cerd{\'a}n},
  journal={Journal of neuroscience research},
  year={2007},
  volume={85 15},
  pages={3244-53}
}
We investigate the mechanisms underlying the redox switch/redox coupling hypothesis by characterizing the competitive consumption of glucose or lactate and the kinetics of pyruvate production in primary cultures of cortical neurons and astrocytes from rat brain. Glucose consumption was determined in neuronal cultures incubated in Krebs ringer bicarbonate buffer (KRB) containing 0.25-5 mM glucose, in the presence and absence of 5 mM lactate as an alternative substrate. Lactate consumption was… CONTINUE READING