Kinetic properties of human dopamine sulfotransferase (SULT1A3) expressed in prokaryotic and eukaryotic systems: comparison with the recombinant enzyme purified from Escherichia coli.

@article{Dajani1999KineticPO,
  title={Kinetic properties of human dopamine sulfotransferase (SULT1A3) expressed in prokaryotic and eukaryotic systems: comparison with the recombinant enzyme purified from Escherichia coli.},
  author={Rana Dajani and Starlette Sharp and Shirley Graham and Susanne S. Bethell and R. M. Cooke and Derek J. Jamieson and Michael W. H. Coughtrie},
  journal={Protein expression and purification},
  year={1999},
  volume={16 1},
  pages={11-8}
}
Sulfation, catalyzed by members of the sulfotransferase enzyme family, is a major metabolic pathway which modulates the biological activity of numerous endogenous and xenobiotic chemicals. A number of these enzymes have been expressed in prokaryotic and eukaryotic systems to produce protein for biochemical and physical characterization. However, the effective use of heterologous expression systems to produce recombinant enzymes for such purposes depends upon the expressed protein faithfully… CONTINUE READING