Kinetic performance limits of constant pressure versus constant flow rate gradient elution separations. Part II: experimental.


We report on a first series of experiments comparing the selectivity and the kinetic performance of constant flow rate and constant pressure mode gradient elution separations. Both water-methanol and water-acetonitrile mobile phase mixtures have been considered, as well as different samples and gradient programs. Instrument pressures up to 1200 bar have… (More)
DOI: 10.1016/j.chroma.2010.12.087