Kinetic modelling of the proton translocating CF0CF1-ATP synthase from spinach.

@article{Pnke1996KineticMO,
  title={Kinetic modelling of the proton translocating CF0CF1-ATP synthase from spinach.},
  author={Oliver P{\"a}nke and Brandon Rumberg},
  journal={FEBS letters},
  year={1996},
  volume={383 3},
  pages={196-200}
}
The rate of both ATP synthase and hydrolysis catalysed by the thiol-modulated and activated ATP synthase from spinach is measured as a function of all substrates including the protons inside the thylakoid lumen. The most important findings are: (1) sigmoid kinetics with respect to H+in, (2) hyperbolic kinetics with respect to ADP, ATP and phosphate, with Km for phosphate and ADP decreasing upon increasing H+in, (3) binding of ADP and phosphate in random order and competitive to ATP. Simulation… CONTINUE READING