Kinetic model of in vivo folding and inclusion body formation in recombinant Escherichia coli.

@article{Hoffmann2001KineticMO,
  title={Kinetic model of in vivo folding and inclusion body formation in recombinant Escherichia coli.},
  author={Frank Hoffmann and Clemens H. Posten and Ursula Rinas},
  journal={Biotechnology and bioengineering},
  year={2001},
  volume={72 3},
  pages={
          315-22
        }
}
Aggregation of misfolded proteins can reduce the yield in recombinant protein production. The underlying complex processes are additionally influenced by cellular physiology. Nevertheless, a lumped-parameter model of kinetic competition between folding and aggregation was sufficient to track properly the specific concentration of a human protein produced in E. coli and its partitioning into soluble and insoluble cell fractions. Accurate estimation of the protein-specific parameters required… CONTINUE READING
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