Kinetic method having a linear range for substrate concentrations that exceed Michaelis-Menten constants.

@article{Hamilton1982KineticMH,
  title={Kinetic method having a linear range for substrate concentrations that exceed Michaelis-Menten constants.},
  author={Steven D. Hamilton and Harry L. Pardue},
  journal={Clinical chemistry},
  year={1982},
  volume={28 12},
  pages={2359-65}
}
We describe a new data-processing method for the kinetic quantification of substrates of enzyme-catalyzed reactions. Nonlinear regression is used to fit data for absorbance (A) and rate (dA/dt) vs time to the rate form of the Michaelis-Menten equation. Fitting parameters are the maximum velocity (Vmax), the Michaelis constant (Km), and the total absorbance change (delta A infinity) that would be observed if the reaction were monitored to completion. The method is evaluated with use of the… CONTINUE READING