Kinetic mechanisms of glycine oxidase from Bacillus subtilis.

  title={Kinetic mechanisms of glycine oxidase from Bacillus subtilis.},
  author={G. Molla and Laura Motteran and Viviana Job and Mirella S. Pilone and Loredano Pollegioni},
  journal={European journal of biochemistry},
  volume={270 7},
The kinetic properties of glycine oxidase from Bacillus subtilis were investigated using glycine, sarcosine, and d-proline as substrate. The turnover numbers at saturating substrate and oxygen concentrations were 4.0 s(-1), 4.2 s(-1), and 3.5 s(-1), respectively, with glycine, sarcosine, and D-proline as substrate. Glycine oxidase was converted to a two-electron reduced form upon anaerobic reduction with the individual substrates and its reductive half-reaction was demonstrated to be reversible… CONTINUE READING