Kinetic mechanism of Escherichia coli isocitrate dehydrogenase.

  title={Kinetic mechanism of Escherichia coli isocitrate dehydrogenase.},
  author={Antony M Dean and Daniel E. Koshland},
  volume={32 36},
The kinetic mechanism of the NADP-dependent isocitrate dehydrogenase of Escherichia coli was investigated using initial steady-state kinetic analyses. Kinetic coefficients, obtained using natural and alternative substrates with the wild-type and two mutant enzymes (S113L and S113N), suggest that the forward reaction [the oxidative decarboxylation of (2R,3S)-isocitrate by NADP] of the wild-type enzyme is a steady-state random mechanism, with catalysis more rapid than product release. The… CONTINUE READING

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