Kinetic mechanism and regulation of myosin VI.

  title={Kinetic mechanism and regulation of myosin VI.},
  author={Enrique M. De La Cruz and E Michael Ostap and H Lee Sweeney},
  journal={The Journal of biological chemistry},
  volume={276 34},
Myosin VI is the only pointed end-directed myosin identified and is likely regulated by heavy chain phosphorylation (HCP) at the actin-binding site in vivo. We undertook a detailed kinetic analysis of the actomyosin VI ATPase cycle to determine whether there are unique adaptations to support reverse directionality and to determine the molecular basis of regulation by HCP. ADP release is the rate-limiting step in the cycle. ATP binds slowly and with low affinity. At physiological nucleotide… CONTINUE READING
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