Kinetic measurements of Escherichia coli RNA polymerase association with bacteriophage T7 early promoters.

@article{Dayton1984KineticMO,
  title={Kinetic measurements of Escherichia coli RNA polymerase association with bacteriophage T7 early promoters.},
  author={C J Dayton and Dennis E. Prosen and Keith L. Parker and Carol L. Cech},
  journal={The Journal of biological chemistry},
  year={1984},
  volume={259 3},
  pages={
          1616-21
        }
}
During infection of Escherichia coli by bacteriophage T7, E. coli RNA polymerase utilizes only three promoters (A1, A2, and A3). In vitro, the A promoters predominate at very low polymerase concentration, but at higher polymerase concentration the minor B, C, D, and E promoters are used with equal efficiency. The binding constant for the initial association of polymerase with promoters and the forward rate of isomerization to an "open" complex capable of initiation have been measured for the A1… CONTINUE READING

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