Kinetic intermediates of unfolding of dimeric prostatic phosphatase.

@article{Kuciel2007KineticIO,
  title={Kinetic intermediates of unfolding of dimeric prostatic phosphatase.},
  author={Radosława Kuciel and Aleksandra Mazurkiewicz and Paulina Dudzik},
  journal={Acta biochimica Polonica},
  year={2007},
  volume={54 2},
  pages={
          371-7
        }
}
Kinetics of guanidine hydrochloride (GdnHCl)-induced unfolding of human prostatic acid phosphatase (hPAP), a homodimer of 50 kDa subunit molecular mass was investigated with enzyme activity measurements, capacity for binding an external hydrophobic probe, 1-anilinonaphtalene-8-sulfonate (ANS), accessibility of thiols to reaction with 5,5'-dithiobis(2-nitrobenzoic acid) (DTNB) and 2-(4'-maleimidylanilino)naphthalene-6-sulfonate (MIANS) and ability to bind Congo red dye. Kinetic analysis was… CONTINUE READING

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