Kinetic immunochemical method for the simultaneous quantification of creatine kinase isoenzymes.

Abstract

We describe the development and evaluation of a kinetic immunochemical method for the simultaneous quantification of isoenzymes. Specifically, we use the inhibition of the M subunit of creatine kinase (CK; EC 2.7.3.2) by antibodies to quantify isoenzymes CK-MM and CK-MB in serum matrices. Nonlinear least-squares data-processing is used to compute the enzyme… (More)

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